permanent mount, stiff cardboard slide,
glass slide, coverslip, eye dropper, thin piece of tule and cattail (or other
appropriate material), white glue, transmitting and reflecting light
Look at Permanent
Mount with both a transmitting and light microscope. Which one has
the ability to see in more detail?
Dry Mount -
Comparing tules and cattail structure. Use a cardboard slide and use
white glue to attach them. Label and draw what you see below.
Use a reflecting light microscope.
a Wet Mount
Place slide on a flat surface.
Place a drop of water on the slide, including the organism that you
are using. If what you are looking at is inanimate, put the
object down first than put water.
Hold the coverslip by its sides and lay its bottom edge on the slide
close to the specimen. You may want to use a forcep for better
control. Holding the coverslip at a 45E
Slowly lower the coverslip so it pushes air bubbles out as the water
spreads under the coverslip. If you get air bubbles (looking like little
black doughnuts), gently press on the coverslip to move them to the edge. If
there are dry areas under the coverslip, add a little more water at the edge
of the coverslip. Too much water can be dabbed off with a piece of paper
Use a transmitting light
microscope. Describe what you see, especially the clarity of the slide.
Are there air bubbles?
Are there void spaces?
This might be because you don’t have enough water.
Make a Live Mount
using both the “well” and the demoslide. When you put
material in the demoslide you will need to remove the air bubbles by snapping
your finger along the side several times. Use a
reflecting microscope to view the well, and transmitting light microscope for